Clarence Yapp

In this video, we demonstrate how optoporation can be used to direct drugs into cells for drug development and screening. Here, DAPI, a blue fluorescent stain that fluoresces when it binds to DNA, will mimic a drug. Generally, DAPI is impermeable to living cells and is normally used to stain for dead cells.

This timelapse video shows a human fibroblast cell being optoporated in the presence of DAPI. After optoporation of the fibroblast's cell membrane, DAPI travels into and throughout the cell from the point of entry. Because DAPI only stains DNA, it will only bind, and therefore fluoresce, in the nucleus, and not the cytoplasm where DNA is absent.

Interestingly, one may be able to observe that the cell shrinks by a small amount. This could be due to some of the contents of the cell leaking out. Меньше