Well, I suppose I just couldn't stay away. Thanks to all those who sent me such kind words of support and encouragement in both comments and PMs. It's likely that without them this video would not exist. I've enjoyed my break, and I think I've learned the value of taking things a little easier. These videos take an incredible amount of time and effort to make, and I think I will be easing off the accelerator in the future. Thus while it's likely that this won't be the last HH, please be patient with me as the gaps between episodes will probably be a lot larger than you've been used to in the past. Thank you for your patience and, as ever, your kind and undeserved support.
Anyway, for this episode, I thought it most appropriate to sodomize the PPSimmons channel again, since it appears that they neglected to accept my counter with regards to their disingenuous debate offer. I think it is highly fitting, therefore, that Pastor Carl's anus pay the price for their cowardice. You can find the stain to which I'm responding here:
http://www.youtube.com/watc...The eccentric-gentleman-at-the-end
-of-the-video's channel can be found here:
http://www.youtube.com/user...
Scripts to this and all my other videos can be found here:
http://thelivingdinosaur.we...
Intro by the one-and-only ONESPECIES (check him out too!):
http://www.youtube.com/ONES...
"Endless forms most beautiful" quote courtesy of Mr. C. Darwin.
Music: Wolfgang Amadeus Mozart, Symphony No. 19 in E flat major (K 132)
References:
Jermutus, L., Honegger, A., Schwesinger, F., Hanes, J., and Plückthun, A. (2001). Tailoring in vitro evolution for protein affinity or stability. Proc. natl. Acad. Sci. USA. 98: 75--80.
Kaltenbach, M. and Hollfelder, F. (2012). SNAP display: in vitro protein evolution in microdroplets. Methods Mol. Biol. 805: 101-11.
Kashiwagi, K., Isogai, Y., Nishiguchi, K-.I., and Shiba, K. (2006). Frame shuffling: a novel method for in vitro protein evolution. Protein Eng Des Sel., 19: 135-40
Lipovsek, D. and Plückthun, A. (2004). In-vitro protein evolution by ribosome display and mRNA display. J. Immunol. Methods. .290: 51-67.
Moore, J.C. , Jin, H-.M., Kuchner , O. and Arnold, F.H. (1997). Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of Improved Sequences. J. Mol. Biol., 272: 336-47.
Nishikawa, T., Sunami, T., Matsuura, T. and Yomo, T. (2012). Evolution of proteins through in vitro protein synthesis in liposomes. doi: 10.1155/2012/923214.
Soramoto, S., Ueno, S., Tabuchi, I. and Husimi, Y. (2002). Design of various high quality random libraries for in vitro protein evolution. Genome Informatics, 13: 527-8.
Tabuchi, I., Soramoto, S., Nemoto, N. and Husimi, Y. (2001). An in vitro DNA virus for in vitro protein evolution. FEBS Lett., 508: 309-12.
Voigt, C.A., Kauffman, S. and Wang, Z.G. (2000). Rational evolutionary design: the theory of in vitro protein evolution. Adv. Protein. Chem. 55: 79-160.
Wackett, L.P. (1998). Directed evolution of new enzymes and pathways for environmental biocatalysis. Ann. N.Y. Acad. Sci. 864: 142-52.
Yuan1, L., Kurek, I., English, J. and Keenan, R. (2005). Laboratory-directed protein evolution. Microbiol. Mol. Biol. Rev., 69: 373-92.
Seelig, B. and Szostak, J.W. (2007). Selection and evolution of enzymes from a partially randomized non-catalytic scaffold. Nature, 448: 828-31.
Chao, F.-A., et al. (2012). Structure and dynamics of a primordial catalytic fold generated by in vitro evolution. Nature Chem. Biol., 9: 81-83.
Singh, R.K. et al. (2013) From protein engineering to immobilization: Promising strategies for the upgrade of industrial enzymes. Int. J. Mol. Sci., 14, 1232-77.
Tobe, S. et al. (2006). Expression of Bacillus protease (Protease BYA) from Bacillus sp. Y in Bacillus subtilis and enhancement of its specific activity by site-directed mutagenesis-improvement in productivity of detergent enzyme. Biol Pharm Bull., 29: 26-33.
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