Watch Queue
Queue
Watch QueueQueue
The next video is starting
Loading...
-
-
-
-
Sign in to like videos, comment, and subscribe.
Watch Queue
Queue
__count__/__total__
-
protein preparation
A basic run through of a standard NiNTA his tag protein purification-
protein prep spinning down cells.3gp - Duration: 8 minutes, 32 seconds.
-
protein prep - pouring of supernatant and breaking down cell pellet.3gp - Duration: 4 minutes, 9 seconds.
-
protein prep - resuspending cell pellet into pbs and lysozyme.3gp - Duration: 2 minutes, 41 seconds.
-
protein prep - pouring resuspention into beakers for sonication.3gp - Duration: 2 minutes, 36 seconds.
-
sonication - placing probe and starting sonication.3gp - Duration: 93 seconds.
-
protein prep - Putting our lysate into falcon tubes.3gp - Duration: 2 minutes, 5 seconds.
-
protein prep - balancing lysate samples for centrifugation.3gp - Duration: 3 minutes, 6 seconds.
-
protein prep - setting up the centrifuge and spinning the lysate down.3gp - Duration: 105 seconds.
-
protein prep - first spin down of lysate and transfer to new tube.3gp - Duration: 2 minutes, 43 seconds.
-
protein prep - second spin, making lysate mixtures , and adding ni-nta resin.3gp - Duration: 12 minutes, 58 seconds.
-
protein prep - pouring beads onto flwo column.3gp - Duration: 6 minutes, 20 seconds.
-
protein prep - washing resin bed, making PBS and making elution buffer.3gp - Duration: 8 minutes, 59 seconds.
This item has been hidden -
-
cloning cycle
-
the cloning cycle part 1 - Duration: 9 minutes, 57 seconds.
-
cloning cycle part 2 - Duration: 8 minutes, 58 seconds.
-
biobricks standard assembly part 1 - Duration: 9 minutes, 53 seconds.
-
biobricks standard assembly part 2 - Duration: 8 minutes, 36 seconds.
-
cloning cycle - how to set up a resctriction digest on plasmid or pcr DNA - Duration: 9 minutes, 47 seconds.
-
cloning cycle runing a gel of our digestion - Duration: 7 minutes, 26 seconds.
-
cloning cycle imaging and cutting DNA agarose gels - Duration: 7 minutes, 6 seconds.
-
cloning cycle melting gel fragments for dna purification - Duration: 98 seconds.
-
cloning cycle agarose gel DNA purification with zymo columns pt1 - Duration: 8 minutes, 53 seconds.
-
cloning cycle agarose gel DNA purification with zymo columns pt2 - Duration: 6 minutes, 58 seconds.
-
cloning cycle how to set up a ligation - Duration: 6 minutes, 10 seconds.
-
cloning cycle transforming and plating a ligation - Duration: 9 minutes, 31 seconds.
This item has been hidden -
-
transformation by electroporation
-
datsenko wanner genome integration - reseeding cells for electroporation - Duration: 4 minutes, 40 seconds.
-
datsenko wanner induction for electroporation - Duration: 62 seconds.
-
datsenko wanner electroporation spin down culture - Duration: 4 minutes, 41 seconds.
-
datsenko wanner electroporation toss supernatant, resuspend in 10% glycerol, and second spin - Duration: 4 minutes, 16 seconds.
-
datsenko wanner electroporation toss glycerol supernatant, resuspend in 10% glycerol, and third spin - Duration: 3 minutes, 19 seconds.
-
Electroporation tossing supe and resuspending in glycerol and third spin - with camera angle changed - Duration: 3 minutes, 19 seconds.
-
datsenko wanner electroporation toss glycerol supernatant, final resuspend in 10% glycerol, and prep for electroporation - Duration: 2 minutes, 20 seconds.
-
datsenko wanner electroporation the actual electroporation - Duration: 6 minutes, 56 seconds.
-
electroporation the actual transformation - performed a second time - Duration: 10 minutes, 45 seconds.
-
electroporation plating a library with beads and titering a library using a serial dilution - Duration: 9 minutes, 37 seconds.
This item has been hidden -
-
Uploads
-
Lab automation - just watching the robot do its thing - Duration: 3 minutes, 26 seconds.
-
Lab automation - adjusting inking depth and picking - Duration: 7 minutes, 32 seconds.
-
Lab automation - colony picking robot - setting up how the robot chose colonies to pick - Duration: 9 minutes, 59 seconds.
-
Lab automation - colony picking robot - calibrating the robot - Duration: 8 minutes, 6 seconds.
-
Lab automation - colony picking robot setup - Duration: 8 minutes, 56 seconds.
-
Lab automation - filling up 384 well plates with media - Duration: 5 minutes, 5 seconds.
-
Lab automation - Using a Biomek to distribute media into 384 well plates - Duration: 11 minutes, 23 seconds.
-
Lab automation - cherry picking from and to 384 well plates - Duration: 6 minutes, 22 seconds.
-
Looking at the result of a spotting assay - Duration: 3 minutes, 58 seconds.
-
01 Colony PCR with master mix part 1 - Duration: 14 minutes, 40 seconds.
-
02 Colony PCR with master mix part 2 - Duration: 111 seconds.
This item has been hidden -